Abstract: The reproductive development of male inflorescence for Betula platyphylla takes almost a whole year from the emergence of original male inflorescence to the formation of mature pollen. The early and middle development periods are much essential for the development of male gametophyte of B. platyphylla. The differentially expressed transcripts in early and middle developmental stages were analyzed by cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique. Among 62 successfully sequenced fragments, 30 showed significant homologies to known genes in Genbank, while the other 32 were supposed to unknown and new sequences. GO annotation showed that these known genes were involved in the processes of metabolic process, cellular process, response to stimulus, reproduction, signal transduction, and biological regulation, and then with the molecular function of catalytic activity, binding molecular, enzyme regulator activity and transporter activity. Two important TDFs involved in reproduction and male inflorescence development of B. platyphylla were found, named as Bplbs658 and Bplbs199. In addition, the transcript expression of 11 TDFs were examined in different tissues by qRT-PCR. The results indicated that these genes were involved in the development of both floral tissues and vegetative tissues of B. platyphylla with distinct tissue-specific expression patterns.