Abstract: Applying RT-PCR and RACE method, 3 142 bp pepc gene was isolated from Jatropha curcas. The sequence analysis showes that the pepc gene including an open reading frame is 2 898 bp in length, and encodes a protein of 965 amino acid. Its amino acid sequence shares 94.94%, 92.46%, 90.60%, 90.50%, 90.50%, 88.33%, 84.61%, 82.44% identity with those of pepc from Ricinus communis, Gossypium hirsutum, Citrus sinensis, Glycine max, Arachis hypogaea, Nicotiana tabacum, Brassica napus, Arabidopsis thaliana, respectively. The pepc gene has been accepted by GenBank, and the accession number of gene sequence is EU069413. The deduced protein molecular weight of pepc is 110.6 kD, and belong to pepc1 in gene family and C3 form. Its stability, secondary structure, hydrophobicity profile were analysed, and the functional sites and characterized domains were confirmed.