松墨天牛纤维素酶的研究 Ⅱ.离体条件下金属离子对松墨天牛纤维素酶活性的影响

Study on the Character of Cellulase in Monochamus alternatus Ⅱ.Effect of Some Ions on the Monochamus alternatus Cellulase Activity in Vitro

摘要: 本文研究了Cu++、Mn++、Ca++、Pb++、Ag+、Zn++、Na+、Fe++8种金属离子对松墨天牛(幼虫、成虫)消化道纤维素酶活性(离体条件下)的影响。结果表明:不同的金属离子对纤维素酶有不同程度的影响,除Fe++有激活作用外,其它7种金属离子对纤维素酶各组分酶活性在实验所取浓度范围内(0.150 mmol.L-1)表现为程度不同的抑制作用,其中Cu++和Pb++的抑制效应最强,这在纤维素酶的组分(Cx酶、-βl,4-葡萄糖苷酶、C1酶)都有很鲜明的体现,与对照相比较,在不同浓度梯度下使Cx酶的活性下降13.19%99.98%和42.27%100%。

Abstract: The effects of eight ions on the Monochamus alternatus cellulase activity in vitro were studied in this paper.The results indicated that the activity reduced differently when concentration of ions were added except Fe++ in experiment conditions.Among which the effects of Cu++ and Pb++ were obvious on cellulolytic enzyme complex: endo-β-1,4-glucanase(Cx-ase),exo-β1,4-glucanase(C1-ase) and β-1,4-glucosidase in their gut,and activity had declined by 13.19%～99.98% and 42.27%～100% respectively under different concentration gradients.