Abstract: A study has been made of isolating isoenzymes of POD and EST of Zizyphus mauritiana from 5 provenances with discontinuous polycrylamide gel system (Disc PAGE).The results have shown that there are differences present on the spectrum of the two isoenzymes in the tested plants.EST is more sensible to tell their relationships close or distant,and the differences in the Burma and Yunnan Provenances appear more obvious,while the Shuangbei and Yuanmao ones show no significant differences.And it needs further study if the latter two come from the same provenance.