Abstract: In order to study the effect of plant growth regulators on the healing progress of the nurse seed grafted unions of Camellia oleifera, an orthogonal experiment of GGR6, IBA, Brassinolide, different concentration and application method (leaching medium, dunking root, and spraying leaves) on the nurse seed grafted seedlings of C. oleifera was carried out. The paraffin sections and microscopic observation system were used to measure the area of the callus and grafted union and get the healing rate, then the healing progress was made to be measurable. The results showed that the application of plant growth regulators significantly promoted the healing progress, the form of the callus and the connection of stock and scion. The average healing rate of different treatment was 28.01% higher than that of the control group, and the time required for the grafted union to be healed was 15 days less than the control group. The effect of different concentrations and application methods of plant growth regulators on the healing progress and survival rate was significantly different, among these treatments, the best was 0.03 g·L-1 GGR6, 0.2 g·L-1 IBA and 0.14 mL·L-1 0.28 mL·L-1 Brassinolide mixed liquid to leach medium 1.42 L per 500 mL.