Abstract: With the Styrax tonkinensis from Jishui, Jiangxi Province, the morphological structure of pollen was observed under SEM. The storage methods determining the pollen viability were compared among culture in vitro, I2-KI staining and TTC staining. Meanwhile, the effects of different storage condition on pollen germination was investigated. The results show that the pollen of S. tonkinensis with microreticulate ornamentation in outer wall is 3-colpate and prolate. The average polar and equatorial axis of the pollen is 52.33±0.577 μm and 26.89±0.502 μm. The method of culture in vitro is the most effective to the determination of pollen viability. The best combination for pollen germination is 10% sucrose, 0.05 g·L-1 boric acid, 100 mg·L-1 GA3, 3 h. -80℃ sealed storage is the best storage condition to keep the vitality of pollen. After 180 days'storage, the germination rate of the pollen is still as high as 40.97%.