Abstract: Tests for fitting the culture medium for Verticillium chlamydosporium which had high parasitic rate on Meloidogyne incognita eggs were bioassayed.The experiment studied on the influence of different carbon source and nitrogen source,initial pH value,inorganic salts on the sporulation by observing the spore output every 12 hours.Under the culture condition of temperature 28 ℃,200 mL inventory per 500 mL flask and speed of agitation 160 r·min-1,the optimal fermentation medium was obtained which included glucose 20 g·L-1,corn flour 20 g·L-1,soybean powder 20 g·L1,KH_2PO_4 0.5 g·L-1,MgSO_4·7 H_2O 0.5 g·L-1.The best initial pH value ranged from 5.0 to 7.0 and the optimum time for fermentation was 120～144 h.