Abstract: Objective A 25-year-old Eucalyptus cloeziana provenance trial was conducted in Guangxi Dongmen Forest Farm to analyze the genetic variation of the growth and stem form quality traits in order to select the superior provenances and individuals for further study in medium and large diameter of wood and seed breeding.Method The genetic variation of growth and stem form quality traits of 11 E. cloeziana provenances were analyzed and selected by variance analysis and comprehensive analysis of the building of the equation based on the trait selection index. Result The results showed highly significant differences in diameter at breast height (DBH), tree height (H), individual tree volume (V), stem straightness (ST) and stem fullness (SF) among 11 Eucalyptus cloeziana provenances. The heritabilities(H2)of five main traits was 0.634~0.895, and individual heritabilities(h2)was 0.136~0.342, selection index equations of provenances and individuals were built based on DBH, H, V, ST and SF. According to this standard, 4 superior provenances and 13 superior individuals were selected, and the genetic gain of volume reached 11.2% and 29.7% respectively. Conclusion These results indicate that the genetic variation of the five main traits is under a strong genetic control at provenance level and a lower genetic control at individual tree level. In consideration of rapid growth and fine wood property, the provenances D47, S14127, B47 and S12195 are superior materials for further study in medium and large diameter of wood breeding.