Abstract: Objective The study aims at investigating the heat shock protein (hsp) genes and analyze the sequence characterization of Ericerus pela during the peak period of wax-secreting. Method The sequences of hsp10, hsp20, hsp40, hsp60, hsp70, and hsp90 were obtained from the transcriptome database of E. pela. The sequence alignment and phylogenetic analysis were performed. And the mRNA expression patterns of hsp40, hsp60, and hsp90 under different temperature treatments were carried out by using the quantitative real time PCR (qRT-PCR). Result A total of 32 HSPs, which were highly similar with the HSP reported in other species, were identified. Most of them had the conserved regions and characteristic sequences, and they were clustered together with the sequences of the same order insect in the phylogenetic trees. The qRT-PCR results showed that, hsp40, hsp60, and hsp90 can be induced by temperature-stress. Conclusion In this study, 32 unigenes of HSP of E. pela were identified, including 3 hsp10 unigenes, 1 hsp20 unigenes, 11 hsp40 unigenes, 1 hsp60 unigenes, 13 hsp70 unigenes, and 3 hsp90 unigenes.