Abstract: This paper deals with the comparative study on cold resistance of 13 provenances of Black Wattle (Acacia mearnsii) by methods of electro -conductance, growth restoration test, refractometer and tetragolium (TTC) The results showed a similar trend of cold resistance in various provenances. There are three provenances (11, 12, y) with stronger cold resistance and four provenances (16, C1,C2, B) with weaker cold resistance. The characters of these four methods and their application prospect in determination of cold resistance of Black Wattle have been discussed. According to the condition of instrument, it was suggested to determine cold resist ance by an applicable method.