Abstract: Hyphantria cuea nucleopolyhedrovirus (HcNPV) and several other nucleopolyhedrosis was extracted bydifferential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten andanalysed by SDS - PAGE. It indicated thatmost of those p roteins had 32 KD p rotein band, and with 64 KD p rotein,and might be the main p rotein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virusp rotein in 18 KD department had a specific band.