难易生根桉树多酚氧化酶、吲哚乙酸氧化酶活性及其同工酶的比较研究

Comparison on the Activities and Isoenzymes of Polyphenol Oxidase and Indoleacetic Acid Oxidase of Difficult and Easy to root Eucalyptus Species

摘要: 尾叶桉的MLA无性系(简称MLA)是难生根无性系,尾叶桉的U6无性系(简称U6)、刚果12号桉W5无性系(简称W5)为易生根无性系。MLA各器官的多酚氧化酶(PPO)活性比U6、W5的低,而MLA各器官的吲哚乙酸氧化酶(IAAO)活性比U6、W5的高。各树种的PPO活性、IAAO活性及PPO同工酶均具有器官的特异性。讨论了PPO和IAAO与不定根的发生和发展的关系。

Abstract: Eucalyptus urophylla MLA clone (MLA) was difficult-to-root species. Eucalyptus urophylla U6 clone (U6) and Eucalyptus ABL. 12 W5 clone (W5) were relatively easy-to-root species.The activities of polyphenol oxidase (PPO) in MLA is lower than that of U6 and W5,but the activity of indoleacetic acid oxidase (IAAO) in MLA was higher than that of U6 and W5.The activities of PPO and IAAO and the isoenzymes of PPO in every species had their specificity in every organ.The relationship between the formation and development of adventitious roots and PPO and IAAO was discussed.