Abstract: Anthocyanin is a kind of secondary products during the process of flavonoid metabolizing and determine the colors of flowers, fruits and seeds. With the research on flavonoids biosynthesis, many structural genes synthesized anthocyanins of various plants have been cloned, and the previous studies showed that the structural genes were activated by transcription factors (MYB, bHLH and WD40) with complex interaction patterns. In this paper, MYB transcription factors, bHLH transcription factors and WD40 repeat protein involved in anthocyanin biosynthesis and their roles in the regulation of structural gene expression and anthocyanin synthesis are reviewed.