Abstract: This paper reports the influence on nutrient uptake of Eucalyptus urophylla seedlings 5 months after inoculation with Glomus and Pisolithus isolates alone or in competition in a nursery. Though concentrations of some mineral elements in roots or tops varied according to different fungal treatments,there was a substantial increase of nutrient amount of all elements in noculated seedlings. Phosphorus content in inoculated seedlings were 2.67 to 2.88 times of the uninoculated ones. The total content of N,K and B in the inoculated seedlings was respectively 3.58～7.00,2.65～3.18 and 2.78～3.88 folds of those of the uninoculated ones. The results showed that inoculation with Pisolithus and/or Glomus isolates affected nutrient uptake and their distribution in parts of seedlings. The two fungal isolates also influenced the acid phosphatase activity in roots.