Abstract: Objective To study the morphology and development of internal reproductive organs of Tirumala limniace so that to provide theoretical guidance for the artificial breeding of T. limniace. Methods By anatomy method, the morphologies of internal reproductive organs of male T. limniace with ages from one-to twelve-day-old were observed, in the meanwhile, the numerical characteristic parameters were measured, and the development of internal reproductive organs was graded according to the characteristics of each organ so as to understand the morphologies and development of internal reproductive organs of male T. limniace systematically. Results (1) The internal reproductive organs of male T. limniace contain one testis, two vesicula seminalis, a pair of vas deferens, a pair of ejaculatory duct duplex, an ejaculatory duct simplex and accessory gland. (2) With the increase of age, the diameter of ejaculatory duct duplex and the length of ejaculatory duct simplex increased. (3) The length of sperm bundles in testis sustainable increased with the age increased, the length of sperm bundles in vesicula seminalis increased constantly before 7-day-old and tended to stability then. (4) With age increasing, the number of sperm bundles in testis decreased gradually, that in vesicula seminalis increased gradually from 1-to 7-day-old and maintained stability after 7 days, and that in ejaculatory duct duplex increased gradually. (5) The development of internal reproductive organs of male T. limniace could be divided into 4 grades, i.e. milky and translucent stage, transporting and growing of sperm bundle stage, mass transporting and maturing of sperm bundles stage, and replenishing of sperm bundle stage, based on the developmental characteristics of vesicula seminalis and ejaculatory duct duplex. Conclusion The internal reproductive organs of male T. limniace were the same as that of other butterflies; the sperms matured at 6-day-old, and were released to vesicula seminalis and ejaculatory duct duplex in turn, then joined in fertilization. In this study, the developmental process of the internal reproductive organs of male T. limniace was divided into 4 grades for the first time.