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西南桦花粉离体萌发的适宜条件与萌发特征

Prelim inary Report on in V itro Pollen Germ ina tion of B etu la a lnoides

  • 摘要: 应用各种浓度蔗糖、蔗糖+硼酸培养基于系列温度下开展西南桦花粉萌发对比试验,研究了蔗糖、硼酸浓度对西南桦花粉离体萌发的影响,筛选出适合西南桦花粉萌发培养基的适宜组分和适宜培养温度,并应用4个单株的花粉对其萌发特征进行了初步研究。结果表明:蔗糖基本培养基上添加硼酸能够显著促进西南桦花粉萌发;西南桦花粉离体萌发的合适培养基为15%蔗糖+ 200 mg·kg-1硼酸,适宜培养温度为30 ℃;在此条件下培养3 h后花粉萌发率基本稳定,培养7 h后花粉管长度也趋于稳定。

     

    Abstract: In this paper suitable germinatingmedia and optimized temperature for pollens of Betula alnoides Buch. Ham. exD. Don were screened out through comparison tests using all sorts of germinatingmedia with different concentrations of sugarand boric acid under a series of temperature conditions, and its pollen germination processwas characterizedwith pollens collected from four individuals. Itwas indicated that supplementing boric acid to sugarmedia could remarkably accelerate pollengermination of B. alnoides, the suitable media for pollen germination consist of 15% sucrose and 200mg. kg-1 boric acid indistilled water, and optimized temperature was 30 ℃ for this species. And under these good conditions, the pollen germination rate and pollen tube length became stabilized after three hours and seven hours, respectively.

     

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