Abstract: In order to study the bioinformatics characteristics of Populus tomentosa Carr., the local software Blast P was used to search the TUA gene members of Populus in the database of Populus whole genome and Genbank and then the TUA genes family was cloned. By using the software Clustal W and MEGA, the sequence alignment and phylogenetic analysis of TUA genes were conducted. Finally, the TUA protein simulation structures were gotten by the SWISS-MODEL. The result showed that the P. tomentosa α-tubulin genes family consisted of 8 TUA genes members. The 8 members can be divided into two classes, and each class had its own exon-intron property. The three-dimensional structures of the 8 TUA proteins showed extreme similarity. It is proved that the TUA genes have conserve gene and protein structure, and 8 Populus TUA genes are originated from one ancestor.