Abstract:
Objective The aims of this study were to investigate the gene structure and phylogenetic of the mulberry (Morus alba L.) HD-Zip I subfamily members, clarify the tissue-specific expression of these genes in different organs, and reveal the expression level of the family genes under ABA and abiotic stress.
Method Identification of putative HD-Zip I subfamily genes from MorusDB and bioinformatics analysis were performed. The evolutionary tree was constructed based on the multiple sequence alignment of the Arabidopsis and mulberry. RNA-seq data was used to analyze the tissue-specific expression of mulberry HD-Zip I genes. The transcription level of the family genes under hormone and abiotic stress was detected by qRT-PCR.
Result A total of 14 HD-Zip I genes were identified in the mulberry genome. These members could be divided into six classes by phylogenetic tree. Members in the same class were similar in the gene structure and protein motifs. The expression level of both MnHD-Zip 2 and MnHD-Zip 6 were high in root, branch, winter bud, male flower, and leaf according to the RNA-seq data. All the MnHD-Zip I gene except MnHD-Zip 8, MnHD-Zip 9, MnHD-Zip 11, MnHD-Zip 12, and MnHD-Zip 13 were inhibited by ABA. The result of qRT-PCR showed that all the mulberry HD-Zip I genes could be induced by the abiotic stresses.
Conclusion The β clades genes that were highly expressed in most of the organs were observably induced by the NaCl and dehydration. These results indicates that genes from the β clades of MnHD-Zip I play important roles in the development and stress response. In addition, MnHD-Zip 8 and MnHD-Zip 12 were dramatically up regulated by waterlogging, NaCl and dehydration, which indicates that these genes have essential roles in stress regulation of mulberry.
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