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常山胡柚天然色素的初步鉴定及其稳定性研究

Study on In itia l Identif ica tion and Stability of Natural Pigmentof C itrus changshan-huyou

  • 摘要: 以超临界CO2 萃取得到的常山胡柚外果皮色素粗产品为原料,对其进行初步鉴定,并研究了pH值、温度、光照、金属离子、氧化还原剂等对常山胡柚天然色素稳定性的影响。结果表明;常山胡柚天然色素属于黄酮类物质,用NaNO2-Al (NO3 ) 3 比色法进行定量分析得知,色素液中含有黄酮类的量为2. 01 g·L-1 ,该色素在碱性和中性溶液中较稳定,在酸性溶液中不稳定。光照、高温和氧化剂都会对该色素造成破坏;该色素能耐弱还原剂。低浓度Na+的存在会使最大吸光度增加,具有增色作用,高浓度Na+的存在能使最大吸光度降低。随着Zn2+浓度的增加,色素液的色泽明显变淡,吸光度急剧下降。Ca2+ 、Cu2+影响趋势基本一致,加入的离子浓度小于0. 05 mol·L-1时,色素残存率缓慢下降;大于0. 05 mol·L-1时,色素残存率有低幅增加趋势。Mg2+的加入会让色素残存率变小。

     

    Abstract: Initial identification and the effects of pH, temperature, light, metal ions reducing and oxidant onstability of natural p igment, which was extracted by supercritical CO2 extraction from Citrus changshan-huyou wasstudied. The results showed that the p igment was flavonoids, By NaNO2-Al (NO3 ) 3 colorimetric method forquantitative analysis, the flavonoidswas 2. 01 g·L-1 ; the p igment in alkaline and neutral solution wasmore stablewhile in acidic solution the stability was unstable; the light, heat and oxidant could damage the stability; it wasstable under reducing; low concentration of Na+ would increase the maximum absorbance, high concentrations ofNa+ would reduce the maximum absorbance. With the increase of concentration of Zn2+ , the color of p igmentobviously smeared out. The sharp decline in absorbance; Ca2+ , Cu2+ had basically the same impact on the trend.When the concentration was less than 0. 05 mol·L-1 , the residual p igment showed a slow rate of decline, when itwasmore than 0. 05 mol·L-1 , the rate of residual p igment increased in a low amp litude, Mg2+ would diminish therate of residual p igment.

     

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