不同温度处理对‘绿世界’卡特兰花芽分化及内源激素动态变化的影响
The Effect of Different Temperature Treatments on Dynamic Changes of Endogenous Hormones and Flower-bud Differentiation of 'Green World' Cattleya
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摘要: 运用酶联免疫法,测定了卡特兰在花芽分化期经3种不同温度处理其新叶内源激素的动态变化,结果表明:(1)25 ℃/20 ℃处理能显著促进花芽分化,30 ℃/25 ℃处理花芽能正常分化,35 ℃/30 ℃处理抑制花芽分化;(2)25 ℃/20 ℃、30 ℃/25 ℃处理的新生叶片中赤霉素(GA3)、玉米素(ZR)、脱落酸(ABA)含量增加,吲哚乙酸(IAA)含量减少;35 ℃/30 ℃处理抑制新生叶片中GA3、ZR、ABA含量,促进IAA含量,而较低的GA3、ZR、ABA含量和较高水平的IAA含量不利于花芽分化;(3)35 ℃/30 ℃处理使GA3/IAA、GA3/ZR的比值处于一个较为稳定的状态,而这种状态不利于花芽分化。保持较低的IAA/ZR与IAA/ABA水平,有利花芽分化的继续进行。Abstract: The changes of endogenous hormones in the new leaves of Cattleya during the flower-bud differentiation phase with 3 temperature treatments were determined by ELISA. The results indicated that:(1) The flower-bud differentiation was significantly accelerated under the treatment of 25 ℃/20 ℃, which was normal under treatment of 30 ℃/25 ℃ and inhibited under treatment of 35 ℃/30 ℃;(2) The contents of GA3, ZR, and ABA were promoted while IAA was inhibited under the treatments of 25 ℃/20 ℃ and 30 ℃/25 ℃. It was opposite under the treatment of 35 ℃/30 ℃. The low contents of GA3, ZR, ABA and high content of IAA were not good for flower-bud differentiation;(3) The rates of GA3/IAA and GA3/ZR kept stable under the treatment of 35 ℃/30 ℃ which was not good for flower-bud differentiation. Lower rates of IAA/ZR and IAA/ABA was required to keep the flower-bud differentiation continuing.
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