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裂叶垂枝桦组织培养与植株再生

Tissue Culture and Plantlet Regeneration of Betula pendula Roth 'Dalecarlica'

    摘要
  • 摘要:
    目的 建立裂叶垂枝桦组织培养离体繁殖再生体系。
    方法 以裂叶垂枝桦带腋芽或顶芽的茎段为试材，经过外植体消毒、初代培养、继代培养、增殖培养、生根培养，最后获得再生植株，并对裂叶垂枝桦组培快繁影响因素进行分析。
    结果 表明：裂叶垂枝桦幼嫩茎段离体培养最适培养基和激素组合为：MS+0.5 mg·L-16-BA+0.05 mg·L-1NAA+0.2 mg·L-1GA3+20 g·L-1蔗糖+6 g·L-1琼脂；最适生根培养基为：1/2MS+0.1 mg·L-1 NAA+20 g·L-1蔗糖+6 g·L-1琼脂。将生根的无菌苗移植至草炭土和细沙比例3：1的已灭菌的基质中，15 d后，组培苗生长健壮，成活率达到80%以上。
    结论 采用组织培养技术对裂叶垂枝桦进行离体快繁，建立了离体快繁再生体系，为裂叶垂枝桦良种选育奠定了研究基础。

     

    Abstract:
    Objective To establish the tissue culture of vitro propagation regeneration system of Betula pendula Roth 'Dalecarlica'.
    Method Young stems of Betula pendula Roth.'Dalecarlica' with axillary and apical bud were used as experimental materials to obtain regenerated plants by explants disinfection, original culture, successive transfer culture, multiplication culture and rooting culture. The factors influencing the rapid growth of the tissue culture were also analyzed.
    Result The results shows that:the young stem segments in vitro culture for optimum medium and hormone combination was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 NAA+0.2 mg·L-1 GA3+20 g·L-1 Sucrose + 6 g·L-1 Agar. The optimum rooting medium was 1/2MS+0.1 mg·L-1 NAA+20 g·L-1 Sucrose+6 g·L-1 Agar. The rooted seedlings were transplanted to the sterilized matrixes with peat soil and sand in the ratio of 3:1. The tissue-cultured seedlings grew strongly and the survival rate was over 80% after 15 days.
    Conclusion Using tissue culture technique to rapid propagation in vitro for Betula pendula Roth 'Dalecarlica', a rapid propagation in vitro regeneration system was established. It could lay a research foundation for Betula pendula Roth 'Dalecarlica' elite breeding.

     

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