Abstract: The ptc-miR213 was identified from Populus cathayana by microRNA library sequence. It has been confirmed that the ptc-miR213 is induced by salt stress. The putative targets are MYB4, PP2C and protein kinase, in which MYB may play an important role in response to salt stress. To investigate the function of ptc-miR213, the artificial miRNA213 expression vector was constructed and transformed into Arabidopsis thaliana. The transgenic plants were confirmed by PCR and RT-PCR. The results showed that amiRNA transcript level was markedly improved in transgenic plant compared with non-transgenic plants.