Abstract: In order to select the high-efficient in vitro regeneration approaches for transgenic receptor system, the floral organs of Narcissus tazetta var. chinensis ‘Jinzhanyintai’ variety was served as explants and cultured in the MS basic medium. The influence of plant growth regulators on explants type, such as anther, peduncle, ovary and pedicle were studied. The effects of different concentrations of 6-BA, Ad, NAA, 2,4-D and their combinations on callus induction and shoots regeneration from anthers and peduncles were tested by in vitro culture. The results showed that the suitable media for callus induction and shoots regeneration from peduncles were MS + 30 g·L-1 sucrose with 1 g·L-1 NaH2PO4+0.003 g·L-1 6-BA+0.001 g·L-1 NAA+0.2 g·L-1 Ad, and that from anthers were MS+30 g·L-1 with 0.002 g·L-1 6-BA+0.001 g·L-1 NAA. The process of callus from peduncles and differentiation lasted for 30 35 days and the process of callus from anthers and differentiation lasted for 80 90 days. Shoots were regenerated by peduncles callus for histological examination in the process of callus from peduncles and differentiation.