Screening, Identification and Biological Characteristics of Biocontrol Fungi against Larch Shoot Blight

Objective The aim of the experiment is to obtain the biological control strain of larch shoot blight.

Method Strains with strong inhibitory effect on Neofusicoccum laricinum was screened through plate antagonism screening experiments and re-screening with fermentation broth. The screened strains were identified and their biological characteristics were studied.

Result A soil fungi T2 was screened that had a significant antagonistic effect on Neofusicoccum laricinum, which was identified as Trichoderma hamatum by morphology and molecular biology. Its Inhibition rate of plate experiments reached 74.16%, and fermentation broth experiments reached 91.29%. The fermentation broth had strong inhibition effect on a variety of plant pathogenic fungi, and showed strong inhibition effect on Sphaeropsis sapinea, Cytospora chrysosperma, Phyllosticta populea and Rhizoctonia solani with 92.00% inhibition rate and had better inhibition effect on Fusarium sporotrichioides and Marssonina brunnea with 79.67% and 62.67% inhibition rate, too. T2 can utilize a variety of carbon and nitrogen sources, has a wide adaptability to PH and a high lethal temperature.

Conclusion The antagonistic effect of T2（Trichoderma hamatum）on Neofusicoccum laricinum is significant, with a wide spectrum of pathogenic inhibition and strong environmental adaptability. This study can provide theoretical research and foundation for the control of larch shoot blight.