Objective The transcriptome of Amentotaxus argotaenia (Hance) Pilger of Amentotaxus in Taxaceae was sequenced and analyzed for its terpenoid biosynthetic pathway and taxonomy research.
Method The transcriptome of the mixture of stem and leave of A. argotaenia was sequenced by using HiSeq2500.
Result 8.14 Gb clean data was obtained from the transcriptome of A. argotaenia. 82 884 unigene were obtained and 27 495 unigene were annotated using the six public databases. Besides, 2 827 SSRs were identified from the 82 884 unigene. The most abundant type of repeat motif was mono-nucleotides (60.1%), followed by tri-nucleotides (25.4%). Among the unigenes of A. argotaenia, one homologous gene of geranyl diphosphate synthase (GPS), one homologous gene of farnesyl diphosphate synthase (FPS) and five homologous genes of geranylgeranyl diphosphate synthase (GGPS) were identified. In addition, 13 homologous genes to taxadiene synthase gene (TS) in Taxus were obtained using TBLASTN.
Conclusion In this study, 20 unigenes relating to terpenoid biosynthesis and 2 827 SSRs were identified in A. argotaenia. Our work will facilitate the study about the terpenoid biosynthetic genes especially taxadiene synthase gene in A. argotaenia and may provide some foundational genetic data to study its taxonomy and diversity.
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