Screening, Identification and Phosphate Solubilizing Capability of Phosphate Solubilizing Bacteria in Rhizosphere of Camellia oleifera Abel at Red Soil Region

The phosphate solubilizing capability of phosphate solubilizing bacteria (PSB) in rhizosphere of Camellia oleifera Abel apparently affects the Phosphorus use efficiency in red soil. 97 phosphate-solubilizing bacterial strains were isolated from the Camellia oleifera rhizosphere soil of Hu'nan Province, the high effective PSB were further screened using NBRI-BPB medium, and the molybdenum-anti-spectrophotometric method was applied to determine the phosphate-dissolving ability of the high effective PSB after four days' fermentation in NBRIP medium. The bacteria were identified by their morphological, physiological and biochemical characteristics with Biolog system and 16S rDNA gene sequence analysis. The high effective PSB of WB38, WB39 (WB75), WB53 (WB68) were identified as Pseudomonas auricularis, Pectobacterium cypripedii, and Enterobacter ludwigii, respectively. Experiments were conducted to study the phosphate-solubilizing capability of WB38 under different culture conditions. Every factor has a significant influence on phosphate-solubilizing ability of WB38, and the greatest influence factor on phosphate-solubilizing ability was the temperature. The optimum carbon source and nitrogen source were glucose and NH4NO3. WB38 displayed a better phosphate-solubilizing capability at the temperature of 28℃, the medium initial pH was 6.5, the inoculation quantity was 1% and the aeration quantity was 25 mL.