Establishment of Cell Suspension Culture Line and Suspension Culture from the Neem Tree

Studied on the establishment of suspension culture line for the cell of the neem tree (Azadirachta indica) and suspension culture condition. The cell of neem' s suspension cultural line grew best in B5 liquid medium, contained with NAA 2.0～4.0 mg·L-1, inoculation with direct spill method. On the basis of integrative analysis for biomass of cell, content and productivity of azadirachtin, the optimum condition of suspension culture was B5 liquid medium, contained with NAA 2.0～4.0 mg·L-1, saccharose 30 g·L-1, temperature 25 ℃. And according to the size of cultural vessel to decide optimum rotate speed of shake and broth content.