In vitro Culture and Plant Regenera tion of Pa rthenoc issus qu inquefolia

Adventitious shootswere successfully regenerated from cotyledon petioles, hypocotyl exp lants and leaf ex2p lants of in vitro cultures of Parthenocissus quinquefolia. They were cultured on half strengthMurashine and Skoog(MS) , B5 and Heller basalmedium supp lemented with various p lant growth regulators. Auxins, α2naphthaleneace2tic acid (NAA) and in combination with three cytokinins, benzylaminopurine (BA) , kinetin (KT) and thidiazuron( TDZ) were tried. The study showed that the highest regeneration rate were obtained by cotyledon petioles segmentsnear to the hypocotyl under dark time of 30 days cultured on half strengthMS basalmedium supp lemented with 0. 3mg·L -1BA (64. 67%) , hypocotyl exp lants cultured on half strengthMS basalmedium supp lemented with 2. 0 mg·L - 1 KT and 0. 05 mg·L -1NAA and Heller basalmedium supp lemented with 0. 5 mg·L -1BA and 0. 1 mg·L -1NAA ( both about 24% ). Leaf exp lants cultured on B5 basal medium supp lemented with 0. 5 mg·L -1 TDZ and0105 mg·L -1 NAA, the differentiation rate of adventitious budswas as high as 17. 7%. Using half strengthMS asthe multip lication medium for buds supp lemented with 0. 5 mg·L -1 62BA and 0. 1 mg·L -1 NAA, the multip lica2tion coefficient was 3 - 5. Half strengthMS and 0. 5 mg·L -1 IBA and 500 mg·L -1 activated carbon was suitablefor the rooting of regenerated p lantlets, and nearly 100% of these p lantlets survived after transp lanted.