Microdissection and PCR Amplification of Single Cunninghamia lanceolata Chromosome

The single chromosome of Cunninghamia lanceolata was successfully dissected by "glass needles" under the micromanipulator in this research. The dissected Cunninghamia lanceolata chromosomes were digested by proteinase K in Eppendorf respectively. After degenerate olionucleotide primed PCR amplification (DOP PCR), DNA fragments were acquired. The suitable method of making chromosome specimens for the microdissection and PCR amplification has been discussed.