Storage Condition and Viability Change of Camellia japonica Pollen

Fresh pollen of Camellia japonica was used to test the pollen viability by in vitro culture. The effects of different store methods on pollen viability and the activity of SOD, POD and CAT were also investigated. The results show that 1.The best combination for pollen germination was 150 g·L-1 sucrose, 0.05 g·L-1 boric acid, 100 mg·L-1 GA3, and 900 g·L-1 Ca(NO3)2. 2.-80℃ sealed storage was the best storage condition to keep the vitality of the pollen. 3.After 360 days' storage, the activity of the three kinds of enzymes, the antioxidant capacity and aging ability of the pollen kept high. 4.The germination proportion of pollen decreased significantly when the activities of SOD, POD and CAT were around the highest levels. 5.The three enzymes had different responses to the storage temperature. POD and CAT were responding protection enzymes at room temperature, while SOD was a responding protection enzyme at 4℃, however. CAT was a responding protection enzyme at -20℃ and -80℃. The effect of three kinds of enzymes on pollen germination are in the order of SOD>POD>CAT.