Study on the NaCl Concen tra tion and Subculture Times ofBluegra ss Sa lt-toleran t Ca llus Selection

The calluses induced from bluegrass seedswere treated with different concentrations of NaCl to test theirsurvival rate and quality. The results showed that the suitable concentration was 15 g·L -1 forNaCl-tolerant callusselecting. The analysis of survival rate and growth of callus showed that the most op timal subculture timeswere 6 forNaCl-tolerant callus induction of bluegrass under the suitable NaCl concentration. NaCl-tolerant stability test p rovedthat the gained bluegrass callus had steady NaCl-tolerance.