Study on Fast Propagation of Fine Clones of Sequioa sempervirens

In order to propagate and cultivate Sequioa sempervirena,taking 63 clones introduced from France as samples,the study on techniques and measures of tissue culture of Sequioa sempervirens was carried out from the aspects of sample preparing,explant differentiation inducting,bud propagating,and root generating.The results showed that the optimum medium for inducing bud differentiation was MS+6-BA1.5 mg·L-1+KT2.0 mg·L-1,root medium was 1/2MS+IBA1.0 mg·L-1+NAA2.0 mg·L-1.