Genetic Diversity on Walnut Cultivars in Xinjiang Revealed by FISH-AFLP

In order to view the genetic basis of Xinjiang walnut germplasm resourses, the genetic diversity of 30 walnut cultivars (Juglans regia L.) authorized or approved by Xinjiang Uygur Autonomous Region Forest Tree Cultivar Registration Committee were investigated by using FISH-AFLP. Eight pairs of EcoRⅠ+3/MseⅠ+3 primer combinations were used to amplify the genomic DNA. 981 out of totally 1011 AFLP bands were polymorphic and the average percent of polymorphic bands (PPB) was 97.5%. The value of effective number of alleles (Ne) estimated by different primer combinations ranged from 1.188 7 to 1.234 7 with an average of 1.208 5; Nei's gene diversity (H) ranged from 0.118 3 to 0.141 2 with an average of 0.129 7 and Shannon's information index (I) ranged from 0.184 6 to 0.225 8 with an average of 0.206 6, which revealed a moderate level of genetic diversity. Unique fingerprintings for 30 walnut cultivars were established by 8 primer combinations in the research.