Polyphasic Identification of Two Rhizobium Strains from Woody Legumes in Jianfengling of Hainan Province

Two Rhizobium strains RIF200835 and RIF200845 isolated from woody legumes in Jianfengling National Nature Reserve, Hainan province, were analyzed by molecular methods. Their 16S rDNA sequences showed 98.28% and 98.51% of similality to R. miluonense CCUAU 41251T. respectively. The two strains were also investigated for compositions of fatty acid and quinone. The results showed that they were basically similar to the model strain in content but slightly different in components. The DNA G + C contents of the two strains were 63.68% and 60.56%, falling in the range of 57%65% of the genus of Rhizobia. Their housekeep genes (atpD, glnII, recA and rpoB) formed a clade seperating from the model strain R. miluonense CCUAU 41251T. The results showed that the two strains might be new species of Rhizobium.