Abstract: In order to solve the problem of empty holes and bad seedlings in plug tray debase the quality in the process of seedling raising,this paper puts forward to use multiple transplanting hands to replenishing seedlings in proper order and design a multiple transplanting hands replenishing seedling device,which can quickly replenish the seedling in plug tray before leaving the factory and ensure the seedling quality. The performance test of the whole machine was carried out, and the operation object was the seedling of Chinese broccoli of different seedling age.The test results showed that when the seedling age of Chinese broccoli was 16 days, the moving speed of the main mechanism was 0.097 m/s,this device could achieve the ideal production effect, the productivity reached 1028 holes/h,and the success rate reached 96%.