Abstract: Machine vision system is very important for the research and development of harvesting robot. It has a direct impact on the robot’s automatic navigation level and fruit positioning and recognition ability for the performance of machine vision system.In order to realize the harvesting robot from research and development to production experiment,the first step is to design an intelligent machine vision system. Therefore,in the design of the robot navigation machine vision system,it introduced the computer processing system and intelligent PID control algorithm. After adopting the computer processing system,the robot has more powerful image processing ability and control programming ability. With the introduction of intelligent control algorithm,the positioning and navigation of the robot is more accurate,which provided the technical support for the design of the robot automatic navigation system.