Abstract: The trend of grain movement and distribution in the process of threshing and separation has a direct impact on the cleaning load and quality, but it is difficult to observe and study the process of threshing and separation. Therefore, this paper takes Longgu No.31 millet as the research object. According to the structure and branches of the ear, the virtual model of the ear was established by using the binding model of EDEM software. The accuracy of the model is verified by simulation and bench test. The results showed that: the distribution of axial effluents was in accordance with BiDoseResp function, and that of circumferential effluents was in accordance with GaussAmp function. The fitting curve equations of axial and circumferential contrast tests are obtained respectively, and the fitting results are convergent. The results show that there is no significant difference between the simulation results and the bench test results. It is proved that the analysis of threshing and separating process of millet by discrete element method has a high consistency with the actual situation in grain movement and distribution. Discrete element method can be used to study and analyze the threshing and separation process of millet.