Abstract: In order to improve the accuracy of discrete element simulation in the transport process of sunflower seeds, the simulation parameters of discrete element simulation of sunflower seeds were calibrated by combining physical test and simulation test in this paper. Based on the physical test values of contact parameters, Plackett-Burman test was carried out to screen out the parameters that had significant influence on the simulation results. The test results showed that the P values of the impact recovery coefficient of sunflower seed-Q235 steel, the static friction coefficient of sunflower seed-sunflower seed and Poisson’s ratio of sunflower seed were less than 0.05, which had significant influence on the simulation test of Angle of repose. With the test result of Plackett-Burman relative error as an index for the steepest grade test, the optimum parameter value scope; The Box-Behnken test was used to establish the second-order regression equation between the Angle of rerest and the significance parameters of the simulation test. Taking the physical test value as the objective, the optimal parameter combination was obtained: the recovery coefficient of the collision between sunflower seeds and Q235 was 0.33, the static friction coefficient of sunflower seeds and sunflower seeds was 0.42, and the Poisson’s ratio of sunflower seeds was 0.35. Through the verification test and T test to verify the simulation with the Angle of physics test there was no significant difference between Angle of repose. The results show that the optimal parameters obtained can be used for discrete element simulation in the transport process of sunflower seeds, and can also provide a reference for other particle simulation experiments.