Abstract: In order to solve the problem of poor flexibility and stability performance of the crop phenotype information acquisition robot chassis in the field, a crop phenotype information acquisition robot chassis was designed for the cropping pattern and agronomic requirements of wheat. The overall structure and working principle of the chassis were elaborated, the design of the frame was completed, and the design and theoretical analysis of the chassis stabilization mechanism, the design and calculation of the driving and steering components were carried out, and the 3D model was established. ANSYS software simulated the frame, and the analysis results showed that the strength and stiffness of the chassis frame met the requirements of the program design. Finally, the whole machine was tested and field tests were conducted. The field performance test results show that the operating speed of the chassis is 3.6km.h, the minimum turning radius is 1.9m, the straight-line driving offset rate is 1.98%, and the lateral rollover angle is 30.15°, which has good steering flexibility and stability performance and can meet the stable and efficient working requirements of crop characterization information acquisition.