Abstract: Molecular distillation technology was used to separate and purify Xanthoceras sorbifolia Bunge oil, neuronic acid content of recombinant fraction was used as detection index, effects of distillation temperature, feed rate, and scraping speed on its extraction were studied．Test results showed that when optimized process included preheating temperature of 50 °C for raw materials, cooling water of 10 °C, system pressure of 0.1 Pa, distillation temperature of 255 °C, feed rate of 900 g/h, and scraper speed of 350 r/min, Xanthoceras sorbifolia Bunge oil with a purity of 3.37% of neuronic acid could be obtained．