Abstract: Using effects of functional bacteria application cultivated at different temperature gradients and can degrade different organic matter during different composting periods, relationship between substantial change in aerobic composting, odor emission, and composting physical and chemical properties under functional bacteria action was explored．At low, medium, and high temperatures, LB, PDA, and each organic matter screening medium were used to conduct primary and re-screening, and finally functional bacteria that could degrade different organic matter were screened out．Through its reaction circle diameter/strain diameter ratio（D/d）in screening medium, bacterias with stronger organic matter degradation ability were selected, and then through antagonistic reaction to determine whether there is antagonism between bacterias, and finally optimal mixture was combined．Results showed that D/d > 1.5 could prove strong ability to degrade organic matter, and a total of 15 functional bacteria were screened, named DR1～DR15．With a combination of D/d value, antagonistic reaction results of 15 bacterias, and identification results of various organic matter degradation functions, DR3, DR6, DR12, and DR13 four functional bacterias with organic matter degradation ability were finally selected to make bacterial liquid to obtain compound bacterium DR．Compared with control group, when functional bacteria was added to test group, NH₃ emissions increased, which enhanced compost ammonia reaction．H₂S and VOCs emissions were decreased by 35% and 40% respectively, compared with control group．Application of DR to cattle manure composting could effectively reduce H₂S and VOCs emissions in composting process, and all indicators meet composting standards．