细叶石仙桃繁育试验

Propagation Experiment of Pholidota cantonesis Rolfe.

摘要: 以细叶石仙桃荚果为外植体，进行细叶石仙桃再生体系研究。结果表明，用0.1%升汞浸泡8 min细叶石仙桃荚果有利于荚果种子无菌播种，污染率较低；种子萌发培养基为MS＋0.5 mg/L BA＋0.05 mg/L NAA＋7 g/L琼脂时，萌发率高达72%，且原球茎呈色绿成团；诱导增殖培养基为MS＋0.1 mg/L ZT＋30 mL/L椰子汁＋0.05 mg/L NAA＋7 g/L琼脂时，诱导率可达70%，增值系数最高为4.11；在1/2 MS＋50 g/L马铃薯＋50 mL/L香蕉汁＋1 g/L蛋白胨培养基上，壮苗效果最明显，最大苗宽3.54 mm，植株粗壮。

Abstract: Regeneration system of Pholidota cantonesis Rolfe. was studied by taking Pholidota cantonesis Rolfe. legume as explants.Results showed that impregnation with 0.1% HgCl2 for 8 min was beneficial to asepsis seeding for Pholidota cantonesis Rolfe. legume and had low pollution rate.When seed germination medium was MS＋0.5 mg/L BA＋0.05 mg/L NAA＋7 g/L agar，germination rate of 72%，PLB color was green and agglomerate.When induced proliferation medium was MS＋0.1 mg/L ZT＋30 ml/L coconut＋0.05 mg/L NAA＋7 g/L agar，induction rate of 70%，maximum increment coefficient was 4.11.When seed germination medium was 1/2MS＋50 g/L potato＋50 ml/L banana juice＋1 g/L peptone，strong seedling effect was the most obvious，maximum width of seedlings was 3.54 mm，and plants were relatively thick.