Abstract: With the development of science and technology, low field nuclear magnetic resonance (LF-NMR) is increasingly used in agriculture. At present, the interpretation of the transverse relaxation time (T2) inversion spectrum stays at the level of the water phase state distribution in the measured sample. It is beneficial to connect the T2 inversion spectrum to the chemical composition of test subject. In order to find such a connection as the substance-oriented interpretation of the T2 inversion spectrum, LF-NMR was applied for 20 ginkgo biloba (Ginkgo Biloba L.) seeds during germination that divided into 10 groups. Temporal observation of ginkgo biloba seeds over the germination process were carried out in vivo using LF-NMR. Their T2 inversion spectra were collected and compared with those from the reference samples that made from seed powder or different mixtures of the main ingredients of ginkgo biloba seeds to explore the forming mechanism of the signal peaks of T2 inversion spectra for a viable interpretation from the perspective of substances. Analysis of the T2 inversion spectra of ginkgo biloba seeds indicated that water in live ginkgo biloba seed could be divided into 4 phase states according to T2, including 2 distinctive bound water of different kinetic activity with transverse relaxation times spiking at T21 and T22, semi-bound water spiking at T23, and free water at T24. The peak T21, T22, T23 of the T2 inversion spectrum of the starch and protein mixed sample and the peak T24 of the starch and oil mixed sample were exactly the same as the corresponding signal peaks of the seed powder sample in terms of peak time. When the material composition and the ratio were completely the same, the peak times of the peak T21-T24 of the T2 inversion spectrum of the seed powder sample were 12.98%, 32.21%, 13.02% and 0% different from those of the fresh seed, respectively. The proportions of peak T21 and T22 are 41.72% and 29.33% less than those of fresh species, respectively, the proportion of peak T23 is 92.26% higher, and the proportion of peak T24 is 91.71% lower. This showed that the seed tissue structure would affect the relaxation time and phase distribution ratio of its internal water to a certain extent. Only from the perspective of material composition, the water in seed was mainly expressed as relaxation time T21, T22, T23 under the influence of starch and protein, and T24 under the influence of starch and lipid. Therefore, it was believed that peak T21 and T22 was the signal of bound water (their phases are different) that mainly adsorbed on starch and protein, peak T23 is the signal of semi-bound water that mainly fettered by starch and protein, and peak T24 is mainly the signal of free water in seed (a small amount derived from lipid). Results of the temporal observation over the germination process found an interesting pattern of change regarding the water phase states in live seeds. While the unit mass signal amplitude of semi-bound water on a monotonous rise and the rest phase states fluctuate over time, the relaxation time of all signal peaks showed an increasing trend on the whole, and there was no significant fluctuation except T21. What's more, 2 new signal peaks that spiking at T2a (around 10 ms) and T2b (over 1 000 ms) developed when a seed approaches the stage of seed-split and the spikes continued to grow ever since. We called them the "prophet spikes" for they foretell an important change in the seed and was about to split. The approach supply a new angle to interpret T2 inversion spectra with chemical and NMR detection principle insights and a new reference for in vivo analysis of chemical composition changes during seed germination based on LF-NMR method.