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6-苄基腺嘌呤对大豆内部水分分布及其生长状态的影响

Effects of 6-Benzylaminopurine on internal water distribution and growth state of soybean

  • 摘要: 为研究大豆发芽过程中加入不同浓度的6-苄基腺嘌呤(6-Benzylaminopurine,6-BA)后大豆内部水分的分布及对其生长状态的影响,以中黄13为研究对象,在相同环境条件下,用浓度分别为0(对照)、1、3、5、7、9 mg/L的6-BA溶液处理大豆。利用低场核磁共振技术(Low Field Nuclear Magnetic Resonance,LF-NMR)对中黄13在浸泡8 h及萌发12、24、36、48、60 h时间点的波谱信息、图像信息进行采集,同时对豆芽的生长状态进行理化指标和形态指标检测。结果显示:与其他处理组相比,浓度为3 mg/L的6-BA溶液处理的大豆的发芽周期、吸水率、平均质量、发芽率、豆芽轴长、轴径各指标表现均为最优;低浓度(1、3 mg/L)6-BA加速大豆蛋白质的水解,导致其渗透势降低,促进大豆蛋白质体吸收更多的水分,进而促使豆芽轴长变长、轴径变粗;高浓度(5、7、9 mg/L)6-BA使大豆细胞壁在细胞的渗透作用下遭到破坏,导致大豆吸水率降低,进而抑制了大豆的生长。该研究从大豆原料的预处理开始对整个加工过程进行了研究,为豆芽的生产加工提供一定的理论支持和数据参考。

     

    Abstract: Soybean sprout is one type of edible vegetable that is cultivated by sprouting soybeans. Taking Zhonghuang 13 as the research object, a series of treatments were carried out using the 6-BA solution with concentrations of 0, 1, 3, 5, 7, and 9 mg/L under the same environmental conditions. The water distribution in soybean was measured to determine the growth state under the different concentrations of 6-BA during soybean germination. The low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) were selected to clarify the effect of 6-BA on the germination of soybean. Three procedures included the LF-NMR, LF-NMR/MRI imaging, and the acquirement of soybean physical, chemical, and morphological indexes. The proton density (PD) image was extracted from the nuclear magnetic signals collected by the Carr-Purcell-Meiboom-Gill (CPMG) spin echo pulse sequence. The results show that the water evolution was visually determined during soybean germination using the LF-NMR PD images. The soybean germination was divided into the imbibition (0-8 h), protrusion (8-24 h), and germination stage (24-60 h). The germination cycle from the soaking to germination varied with the concentration of 6-BA solution in the treatment groups. The germination time of soybean seeds with the 0, 1, and 3 mg/L of 6-BA solution was shorter than that of the rest, where the best was the 3 mg/L concentration. The water content in various phases of soybean was quantitatively characterized by LF-NMR data. According to the relaxation time, the water content of soybean was divided into three kinds: the bound water T21, semi bound water T22, and free water T23. The sub peak was disappeared gradually on the left side of bound water (0.1 ms 

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