Abstract: In order to have a look at the have an impact on of disinfectants on the anaerobic fermentation device of swine manure, a sequence of experiments had been carried out the usage of distinct concentrations (0.02%, 0.1%, and 0.5% by using mass, m/m) of Virkon^TM disinfectant. The outcomes verified that beneath stipulations of improved Virkon^TM stress, the concentrations of TN, NH₄⁺-N, TP, and COD in the effluent of the anaerobic fermentation machine exhibited extraordinary and mentioned increases, accompanied with the aid of a terrific decline in the degradation price of TS and VS. The most CH₄ manufacturing costs had been 63.24 mL The methane manufacturing costs had been 71.63, 73.10, and 38.17 mL/g, respectively. The complete methane manufacturing was once decreased by using 4.48% in the manipulate crew (CK group) and 16.58%. The check groups, which have been uncovered to 2%, 0.1%, and 0.5% Virkon^TM, respectively, exhibited statistically enormous variations (P < 0.001) in their enzyme activities, with the L crew displaying a 16.58% reduce and the H crew displaying an 86.34% decrease. As the publicity time increased, the key enzyme things to do in the take a look at organizations established a tendency to first amplify and then decrease. Among these, the soil enzyme things to do of S-α-GC, S-β-GC, S-ACP, S-NP, and S-CAT in team H exhibited an extensive inhibition (P < 0.05). Furthermore, high-throughput sequencing printed that the Ace index, Chao index, and Shannon index have been markedly diminished in team H relative to team CK for the duration of the anaerobic fermentation stage (P < 0.001). At the phylum level, the first dominant bacterial team used to be the Firmicutes phylum, accompanied by means of the Proteobacteria phylum. The relative abundance of the Firmicutes phylum lowered with the extend of Virkon^TM concentration, whilst the contrary used to be authentic for the Proteobacteria. At the genus level, the prolongation of anaerobic fermentation time was once determined to end result in an upward thrust in the relative abundance of Trichococcus to various degrees, from 0.19% to 0.39% to 2.8% to 4.2%. Conversely, the relative abundance of Smithella was once determined to be drastically suppressed (P < 0.001) in crew H, with an outstanding limit of 91% in assessment to that determined in crew CK. The PICRUSt2 practical prediction evaluation indicated that the variations in COG practical composition of microbial communities throughout experimental corporations have been no longer statistically significant. Amino acid transport and metabolism, as properly as unknown functions, had been the predominant functions.Combined with the results of KEGG orthology (KO) and the analysis of genes related to KEGG (kyoto encyclopedia of genes and genomes) database, it was found that high concentration of Virkon^TM significantly inhibited the growth and metabolic activity of microbial communities and weakened the methane synthesis efficiency of the anaerobic fermentation system, but on the other hand, it induced an overresponse to the expression activity of the key functional gene K00531 (anfG). Compared with the control group (CK), the surge was about 272 times. The significant upregulation of its expression levels may improve the degradation efficiency of the system for the typical chlorinated contaminants (chlorinated alkanes and chlorinated olefins) in Virkon^TM disinfection by-products by enhancing the activity of key enzymes.