Abstract: Lighting is one of the critical environmental indicators in the intensive pig production. There is the significant influence on the growth, reproductive performance, and behavioral patterns of the replacement gilts. This study aims to identify the optimal protocols of the artificial lighting. Four lighting treatments were also evaluated, including the L1 (white light, 40-50 lx), L2 (white light, 100-120 lx), L3 (green light, 100-120 lx), and L4 (white light with gradually varying intensity). Sixteen replacement gilts with an initial body weight of (86.00±9.50) kg were randomly assigned with four lighting treatments (4 pens per unit, 1 gilt per pen) for a 35-day trial. Body weight and feed consumption were measured on days 1 and 35. The data was then used to calculate the average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F:G). Blood samples were collected to analyze serum hormone levels, including the reproductive hormone (GnRH), estradiol (E2), follicle-stimulating hormone (FSH), progesterone (PROG), cortisol, immunoglobulin A (IgA), luteinizing hormone (LH), and melatonin (MT). Furthermore, the behavioral observations were conducted using video recording systems. Continuous manual recordings were performed from 07:00 to 19:00 on days 10 and 24. The behaviors were also quantified, such as lying, standing, walking, feeding, drinking, defecating, and urinating. The results demonstrated that there was no difference in the growth performance (ADG, ADFI, or F:G) among the four lighting protocols (P > 0.05). However, some significant differences were observed in the physiological and behavioral parameters. L2 (100-120 lx white light) was significantly enhanced the reproductive performance. Compared with the L1, the GnRH levels in the L2 increased by 15.74% (P< 0.05). The E2 levels in the L2 were 16.42% and 14.30% higher than those in the L3 and L4, respectively (P< 0.05). The PROG concentration in the L2 was 29.64% higher than that in the L1. While the FSH levels in the L2 exceeded those in the L4 by 44.98% (P< 0.05). The L2 was enhanced the immune function, with the IgA concentration increasing by 14.72% and 23.44%, respectively (P< 0.05), compared with the L3 and L4. The L4 was reduced the stress responses, with the cortisol concentrations lower than 21.96% and 18.84% L2 and L3, respectively (P< 0.05). The L4 was improved the behavioral functional zoning for the animal welfare. Compared with the L2 and L3, the L4 increased the lying rates on the solid floors by 86.88% and 71.77%, respectively. The urination rates on the slatted floors increased by 69.28%, compared with the L3 (P< 0.05). In conclusion, the lighting protocols were dominated the gilt physiology and behavior. Growth performance was remained on all lighting treatments. White light at 100-120 lx (L2) also shared the most beneficial to the reproductive function. The gradually varying light intensity (L4) was minimized the stress responses to the behavioral organization. The precise production was achieved in the customized lighting strategies. For instance, the white light with 100-120 lx was improved the reproductive capacity of the replacement gilts, or the gradient white light was adopted to enhance the animal welfare levels. This finding can provide the foundational framework for the precision light environment in the commercial pig farming, particularly for the efficiency and animal well-being.