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通过多样化菌剂与环境条件优化千枚岩人造土壤性能

Effects of different microbial agents on artificial phyllite soil quality and properties

  • 摘要: 以往的研究在矿区生态修复方面,生态修复原材料的来源及多尺度时空修复效果等都需要深入探究。为改善秦岭地区生态环境,对秦岭矿区进行生态修复,使用人造土壤对矿坑进行填埋,选择合适的培土原料至关重要,传统的原料为煤矸石,煤矸石为矿物开采的产物,并且培养的土壤中含有重金属元素,不利于生态修复,而千枚岩分布广泛可直接取用,并且无重金属污染。研究通过培土试验、盆栽试验和枯萎试验对培育风化千枚岩人造土壤的最佳条件进行对比。根据风化千枚岩人造土壤质量(土壤容重、土壤pH值、土壤最大持水量和土壤电导率)计算出各组合的土壤质量指数,确定最佳条件,运用方差分析进行处理,排除无效因素,锁定关键因素,提升最佳条件可信度,再经过生物量和发芽率的二维插值验证结果的可靠性。研究发现,经微生物菌剂改良后,风化千枚岩人造土壤的理化性质显著改善,即加入秸秆腐熟剂(JG)60 g用量、培育60 d为最佳处理组合。该处理下的人造土壤容重稳定在1.22 g/cm3附近的理想范围,pH值适中,电导率接近天然土壤。尤为突出的是,其最大持水量高达50.87%,相比天然土壤提升了38%,这直接赋予了土壤优越的保水抗旱性能。盆栽试验进一步验证,该处理组的狗牙根种子发芽率(92%)与生物量均达到最高水平。实地验证发现,覆盖本人工土壤的样方农作物发芽率和生长状态明显优于对比组。研究以“基于自然”的秦岭生态保护和修复等关键科学议题开展,可以为秦岭生态保护修复和生态系统可持续管理的实践需求提供初步理论支撑。

     

    Abstract: Previous studies on ecological restoration in mining areas require further investigation into the sourcing of restoration materials and the restoration effects across multiple temporal and spatial scales. To improve the ecological environment in the Qinling region, ecological restoration of mining areas involves backfilling mine pits with artificial soil. Selecting suitable soil-building materials is crucial. Traditional materials like coal gangue—a byproduct of mineral extraction—contain heavy metals that hinder ecological restoration. In contrast, phyllite is widely available, directly accessible, and free from heavy metal contamination. This study compared optimal conditions for cultivating weathered phyllite artificial soil through soil cultivation experiments, potted plant experiments, and wilting experiments. Soil quality indices (soil bulk density, pH, maximum water-holding capacity, and electrical conductivity) were calculated for each combination of weathered schist artificial soil. Optimal conditions were identified through variance analysis to eliminate ineffective factors and pinpoint key variables, enhancing the reliability of the optimal conditions. The results were further validated using two-dimensional interpolation of biomass and germination rates. The study revealed that microbial inoculants significantly improved the physicochemical properties of weathered schist artificial soil. The optimal treatment combination was adding 60g of straw composting agent (JG) and cultivating for 60 days. Under this treatment, the artificial soil's bulk density stabilized at an ideal range near 1.22g/cm3, with moderate pH and electrical conductivity close to natural soil. Notably, its maximum water-holding capacity reached 50.87%, representing a 38% increase over natural soil, directly conferring superior water retention and drought resistance. Pot experiments further validated that this treatment group achieved the highest Bermuda grass seed germination rate (92%) and maintained high biomass levels. Field verification revealed that crop germination rates and growth conditions in plots covered with this artificial soil significantly outperformed the control group. This research, addressing key scientific issues such as nature-based ecological conservation and restoration in the Qinling Mountains, provides preliminary theoretical support for practical needs in ecological conservation, restoration, and sustainable ecosystem management in the region. The detailed results demonstrated a clear and consistent trend across all measured parameters. Specifically, the bulk density showed a progressive decrease over the cultivation period, eventually plateauing at the optimal 1.22 g/cm3, which is within the ideal range for root growth and water infiltration. The pH values stabilized between 6.5 and 7.2, creating a neutral to slightly acidic environment conducive to nutrient availability and microbial activity. Electrical conductivity remained low, indicating minimal salinity stress, which is critical for plant establishment in restored sites. The remarkable enhancement in maximum water-holding capacity to 50.87% was not only a 38% improvement over local natural soil but also a key factor in reducing irrigation demands and enhancing seedling survival during dry periods. The pot experiments provided robust biological validation; the 92% germination rate observed was accompanied by vigorous seedling growth. Two-dimensional interpolation analysis of biomass and germination data confirmed that the 60g JG 60-day cultivation point represented a clear peak in performance. The integration of these results confirms that the optimized artificial soil formula directly addresses major limitations in mining area restoration, offering a practical, effective, and locally sourced solution that significantly accelerates ecosystem recovery.

     

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