Abstract: Constructing bionic microstructures has been one of the key technologies for achieving wall drag reduction. The drag reduction effects of bionic microstructures with different structures and sizes are different. To explore the drag reduction characteristics of bionic microstructures, three types of closely arranged V-shaped micro-riblet structures (with angles of 60°, 75°and 90°, and a depth of 0.8mm) were designed. The Particle Image Velocimetry (PIV) system was used to observe the flow field changes at different Reynolds numbers. The average flow velocity, Reynolds shear stress and drag reduction effect on the V-shaped micro-riblet wall were analyzed. By conducting spatial correlation analysis, the evolution laws of the turbulent vortex structures on the wall region were explored. The results showed that: 1) The average velocity of the turbulent water flow on the V-shaped micro-riblet wall showed a distinct stratification phenomenon and shifted upward significantly in the log law region. The constant term B value of the fitted average velocity formula increased, indicating that the average flow velocity on the V-shaped micro-riblet wall increased. The average velocity distribution along the V-shaped micro-riblet wall from largest to smallest was 60°, 75°, and 90°, respectively. 2) In the buffer layer, the Reynolds shear stress increased as the riblet angle increased. In the log law layer, the Reynolds shear stress tended to be consistent. Compared with a smooth wall, the Reynolds shear stress of the buffer layer on the V-shaped micro-riblet wall decreased. 3) The characteristic length and structure angle of turbulent vortex structures on the V-shaped micro-riblet wall were smaller compared to those on the smooth wall. The minimum structure angle of turbulent vortex structures was 6.7°. The reduction rate of the characteristic length of vortex structures in the flow direction was controlled within 10%, however, the reduction in the characteristic length of vortex structures in the normal direction is more significant, with a reduction rate ranging from 7.69% to 23.8%, indicating that the V-shaped micro- riblet wall had a strong inhibitory effect on turbulent vortex structures in the normal direction. The V-shaped micro-riblet structures wall altered the spatial correlation of the turbulent water vortices, thereby inhibiting the development of vortex structures. 4) At the condition of the same dimensionless micro-riblet spacing s+, the smaller the angle of bionic riblets, the greater the drag reduction rate. Among them, the maximum drag reduction rates of the 60°, 75° and 90° V-shaped micro-riblet wall were 7.60%, 6.11% and 4.94%, respectively, which proved that the drag reduction rate was greatly affected by the micro-riblet angle. The drag reduction rate of the V-shaped micro-riblet wall of 60° angle was 7.6% within the range of the experiment Reynolds number, which was the best among all the tested riblet structures. The V-shaped micro-riblet wall achieved the effects of speedup and drag reduction by altering the spatial correlation relationship of turbulent vortex structures within the boundary layer. The band-like stripes in riblets inhibited the generation of transient vortex structures and the near-wall turbulence. This paper attempted to apply the bionic V-shaped micro-riblet wall to the water conveyance channel to achieve wall drag reduction. Based on the analysis of the interaction between the turbulent water flow and the bionic wall, the paper studied the drag reduction characteristics and mechanism of the V-shaped micro-riblet wall. Meanwhile, this paper analyzed the characteristics of turbulent water flow changes and the wall drag reduction properties on the V-shaped micro-riblet wall, aiming to increase the average flow velocity on the wall and solve the problem of reduced water conveyance capacity in the irrigation channel caused by the attachment of aquatic plants. The research can lay a theoretical foundation for the application of the V-shaped micro-riblet wall in agricultural irrigation channels.