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精准反馈微波加热对金线鱼鱼糜凝胶特性的影响

Effects of precision feedback microwave heating on gel properties of Nemipterus virgatus surimi

  • 摘要: 为探究精准反馈微波加热(precision feedback microwave heating,PFMH)对金线鱼鱼糜凝胶特性的影响,该研究以金线鱼鱼糜为研究对象,对PFMH在不同温度和加热时间下鱼糜的凝胶强度、持水性(water-holding capacity,WHC)、质构、白度、水分分布、微观结构以及蛋白质二级结构的变化进行分析,并以二段水浴加热作为对照。结果表明,PFMH在85 ℃加热1 min获得的鱼糜凝胶强度与对照组相比提高了58.21%,达到6855.66 g·mm,但80和90 ℃处理的鱼糜凝胶强度均低于对照组。相比于对照组,经过PFMH处理的鱼糜凝胶硬度和黏附性分别提高了30.17%~37.06%和19.82%~25.37%,表明此时鱼糜凝胶的质构特性得以提升;经过PFMH处理的鱼糜凝胶白度下降2.11%~6.44%,但其颜色差异肉眼无法分辨;WHC提升1.25%~9.18%,表明PFMH能够增强蛋白与水的结合,有效地降低水分流动性。低场核磁及其成像结果显示,经PFMH处理的鱼糜凝胶弛豫时间较对照组发生了明显左移,85 ℃加热1 min和80 ℃加热3 min的成像颜色最红,表明其不易流动水占比最高,PFMH能将鱼糜凝胶中的水分锁住,促使水分在其中均匀分布。同时,微观结构结果表明,相比于对照组,PFMH处理的鱼糜凝胶表面平坦,网状结构致密且孔洞有序,蛋白颗粒分布均匀;蛋白质二级结构结果显示,PFMH在85 ℃加热1 min处理的α-螺旋降低至38.22%,β-折叠升高至32.49%,表明其蛋白质二级结构更稳定。该研究采用PFMH对鱼糜进行加热,为改善鱼糜的凝胶品质、减少废水排放,实现绿色低碳化生产,为金线鱼的深加工提供理论参考和技术依据。

     

    Abstract: To investigate the effects of precision feedback microwave heating (PFMH) on the gel properties of Nemipterus virgatus surimi, this study used Nemipterus virgatus surimi as the research subject. The study analyzed the changes in gel strength, water-holding capacity (WHC), textural properties, whiteness, moisture distribution, microstructure, protein distribution, and protein secondary structure of the surimi gels under different temperatures and heating durations conditions of PFMH, with two-stage water bath heating as the control. The results indicated that the gel strength of surimi gels heated by PFMH at 85 ℃ for 1 min increased by 58.21% compared to the control group, reaching 6855.66 g·mm. However, the gel strength of surimi gels treated at 80 and 90 ℃ was lower than that of the control group. Compared with the control group, the hardness and adhesiveness of surimi gels treated by PFMH increased by 30.17%~37.06% and 19.82%~25.37%, respectively, indicating that the texture properties of surimi gels were improved. Although the whiteness of the surimi gels treated with PFMH decreased by 2.11% to 6.44%, the color difference was not discernible to the naked eye. The water-holding capacity of surimi gels treated with PFMH increased by 1.25% to 9.18%, suggesting that PFMH can strengthen the binding between proteins and water, effectively reducing water mobility. Results from low-field nuclear magnetic resonance and imaging revealed that the relaxation times of surimi gels treated with PFMH shifted significantly to the left compared to the control group. The imaging colors of samples heated at 85 ℃ for 1 min and at 80 ℃ for 3 min appeared the reddest, indicating the highest proportion of immobile water. This suggests that PFMH can lock moisture within the surimi gels, promoting a uniform distribution of water. Furthermore, microstructural analysis showed that, compared to the control group, the surface of surimi gels treated with PFMH were smoother, exhibiting a denser network structure and more orderly pores. The surimi gel treated under PFMH at 85 ℃ for 1 min exhibited the smallest pores with uniform distribution. The Df value of the surimi gel heated at 85 ℃ for 1 min was the highest at 1.9695, demonstrating a more compact and uniform microstructure which can effectively retains moisture. Confocal laser scanning microscopy results showed that the protein particles in the surimi gel of the control group were more dispersed, but the protein distribution in the surimi gel treated at 85 ℃ for 1 min was more uniform and the particle size was consistent. Regarding the protein secondary structure, the results showed that PFMH treatment at 85 ℃ for 1 min reduced the α-helix content to 38.22% while increasing the β-sheet content to 32.49%. This indicated that under these conditions, the protein secondary structure became more ordered, effectively facilitating the transition of protein conformations toward a more structured state. This study employed PFMH to heat surimi with the aim of improving its gel quality, reducing wastewater discharge, and achieving green and low-carbon production. The findings provide theoretical references and technical support for the deep processing of Nemipterus virgatus.

     

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