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蒸汽爆破耦合酶法改善槟榔质构的效果与机制

Effect and mechanism of steam explosion combined with enzymatic method on improving the texture of areca nut

  • 摘要: 为明确蒸汽爆破与酶法对槟榔质构的改善及其机制,该研究基于蒸汽爆破以及蒸汽爆破协同酶法处理槟榔,通过测定质构、功能特性及化学成分,结合微观结构和木质素结构表征开展系统分析。结果表明,汽爆处理可破坏槟榔细胞壁结构,使果皮松散、孔隙度增加,但对质构改善效果有限;汽爆 + 酶解耦合处理进一步优化槟榔外观均匀性与纤维分散性,显著降低硬度和咀嚼性、提升弹性,同时大幅提高持水力、持油力和膨胀力。耦合处理使木质素、葡聚糖及木聚糖含量下降,总糖含量升高,且未破坏木质素核心结构,仅改变其分子量分布与聚合状态。综上,蒸汽爆破与酶解耦合处理通过协同破坏槟榔纤维致密结构、适度改性木质素,实现品质的综合提升,为槟榔精深加工提供了理论支撑与技术参考。

     

    Abstract: Areca nut texture can be improved using steam explosion and enzymatic hydrolysis technologies. In this study, a systematic investigation was conducted to clarify the effects of steam explosion–enzymatic hydrolysis on areca nut texture. Textural attributes were examined with microstructure, functional properties, and composition, with an emphasis on lignin structural evolution. A comparison was also performed on a standalone steam explosion and a combined steam explosion–enzymatic hydrolysis. The results demonstrated that better performance was achieved in the steam explosion with enzymatic treatment, particularly with the more uniform color, the low surface wrinkles, and the smoother and flatter morphology. By contrast, steam explosion treatment alone lightened the color of areca nut, surface wrinkles, and the pericarp structure, leading to partial exposure of fibers. In textural properties, the raw areca nut exhibited the highest hardness and chewiness, but the lowest springiness. With steam explosion with enzymatic treatment, the hardness and chewiness significantly decreased by 30% and 8.44%, respectively, while the springiness dramatically increased by 111.4%. The texture indicated that the softer fibrous structure of the product was enhanced oral compliance with low chewing resistance. In terms of functional properties, the raw areca nut showed relatively low water-holding capacity, water swelling, and oil-holding capacity. Steam explosion treatment alone significantly enhanced water- and oil-holding capacity by 15.21% and 29.94%, respectively. The best functional performance was achieved in steam explosion with enzymatic treatment. All three functional indicators were further enhanced, where water-holding capacity, oil-holding capacity, and water swelling capacity increased by 27.38%, 91.62%, and 12.37%, respectively. Scanning electron microscopy observations revealed that the raw areca nut shared a dense cellular structure. After steam explosion treatment alone, cell walls were ruptured with the high porosity. Following the steam explosion with enzymatic treatment, there was even more loose cellular structure, while the cell wall ion was disrupted substantially. With respect to composition, steam explosion treatment alone resulted in minimal lignin, glucan, and xylan content, whereas, the total sugar content increased significantly. After steam explosion with enzymatic treatment, the chemical components decreased significantly, except for the increasing total sugar content. Gel permeation chromatography analysis indicated that steam explosion treatment alone increased the weight-average molecular weight and polydispersity index of lignin, whereas steam explosion with enzymatic treatment was reduced both Mw and PDI. Fourier-transform infrared spectroscopy confirmed that its aromatic structure remained intact after different treatments. Molecular weight distribution was primarily altered to disrupt the polymerization state. Interunit linkages were cleaved to maintain the phenylpropanoid skeleton. Moderate lignin modification was avoided to prevent excessive degradation for product integrity after structure weakening. In conclusion, the steam explosion and enzymatic hydrolysis were combined to synergistically dismantle the dense fibrous architecture of areca nut under physical and biochemical actions. While optimal lignin modification was simultaneously induced to improve the texture, hydration behavior, and overall quality. Thereby, the finding can provide the robust theoretical support and a practical technical reference to advance processing in the areca nut industry.

     

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